GsMTx4 TFA (1209500-46-8 free base) is a spider venom peptide that selectively inhibits cation permeable mechanosensitive channels (MSCs) belonging to the Piezo and TRP channel families.

METHODS: GsMTx4 TFA (5 μM) was tested on outside-out patches of HEK cells transfected with mouse Piezo1 cDNA.
RESULTS GsMTx4 TFA reduced Piezo1-mediated charge transfer to 38% of its initial level. [1]
METHODS: Using C57BL/6 mice as samples, organotypic cerebellar slices (OCS) were prepared and treated with Piezo1 specific activator Yoda-1 (10 μM, 48 hours) or Piezo1 peptide inhibitor GsMTx4 (500 nM, 48 hours). Hours) organotypic cerebellar slices were processed to study how activating or blocking Piezo1 affects myelination.
RESULTS GsMTx4 TFA may rescue psychotin-induced demyelination. [3]
METHODS: MCF10A cells were incubated with adipokines for 24-72 hours. Use GSMTx4 TFA at a concentration of 2.5 μM as a mechanosensitive Ca2+ channel inhibitor for 16 hours. RESULTS GSMTx4 TFA significantly reduced leptin-induced AMPK and MLC-2 phosphorylation. [4]

METHODS: The effects of blocking the Piezo1/CaN/NFAT1 signaling axis by intra-articular injection of GsMTx4 TFA on ACLT-induced osteoarthritis (OA) rats were studied. Taking into account the rapid clearance of synovial fluid, we performed 7, 14, 21, 28 ACLT + H-Gsmtx4 groups at two different frequencies (once a week in the H-GsMTx4 TFA group and once every two weeks in the L-GsMTx4 TFA group). , 35, 42 and 49 days and ACLT + L-Gsmtx4 group were intra-articularly injected with 100 μL of 40 μM Gsmtx4 TFA on 7, 21, 35 and 49 days after surgery.
RESULTS GsMTx4 TFA can improve OA in rats by inhibiting apoptosis and imbalance of anabolism and catabolism. [2]