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JC-1

Catalog No. T15609 CAS 3520-43-2

Synonyms: CBIC2

JC-1 (CBIC2) is a fluorescent lipophilic carbocyanine dye. It has been used to measure mitochondrial membrane potential. JC 1 can be used in a probe for measuring mitochondrial membrane potential by flow cytometry.

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JC-1, CAS 3520-43-2

Pack Size	Availability	Price/USD
1 mg	In stock	$ 59.00
2 mg	In stock	$ 81.00
5 mg	In stock	$ 153.00
10 mg	In stock	$ 252.00
25 mg	In stock	$ 423.00
50 mg	In stock	$ 628.00
100 mg	In stock	$ 893.00

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Purity: 99.58%

Purity: 99.38%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

In vitro

JC-1 fluorescence is usually excited by the 488nm laser wavelength common in flow cytometers[1]. JC-1 (2.5μM) exposed to murine L1210 lymphoblasts, can be detected the presence of both cytoplasmic JC-1 monomer and mitochondrial J-aggregates in these cells. Fluorescent labeling of mitochondria with either JC-1 (1 μg/mL, 15 min), reveals that are distributed irregularly, resulting in regions of high and low mitochondrial content within astrocytes[2]. JC-1 is avidly accumulated in sensitive K562 cells where it displays both a green cytoplasmic and red mitochondrial fluorescence. JC-1 is poorly accumulated in resistant K562 cells, which displays only a slight green fluorescence[4]. JC-1 has been shown to interact with α-synuclein at the acidic C-terminal region with a Kd of 2.6 μM. JC-1 itself does not accelerate the protein aggregation of α-synuclein in the absence of iron, insted, it decelerates the aggregation process by extending the lag phase approx[3].

Synonyms	CBIC2
Molecular Weight	652.23
Formula	C25H27Cl4IN4
CAS No.	3520-43-2

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 14 mg/mL(21.46 mM)

References and Literature

1. A Perelman, et al. JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry. Cell Death Dis. 2012 Nov 22;3:e430. 2. Vera C. Keil, et al. Ratiometric high-resolution imaging of JC-1 fluorescence reveals the subcellular heterogeneity of astrocytic mitochondria. Pflügers Archiv - European Journal of Physiology. 2011,462(5): 693-708. 3. Jung-Ho LEE, In-Hwan LEE, Young-Jun CHOE, et al. Real-time analysis of amyloid fibril formation of α-synuclein using a fibrillation-state-specific fluorescent probe of JC-1. Biochem. J. 2009, 418:311-323. 4. Salvioli S, et al. JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 1997 Jul 7;411(1):77-82.

Citations

1. Wang X, Shi W, Wang K, et al.Rationally Designed Nonapeptides with Great Skin Photoprotective Effect through Producing Type 1 Collagen and Blocking the mTOR Pathway.Journal of Medicinal Chemistry.2023

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

JC-1 3520-43-2 Others inhibit CBIC 2 JC 1 CBIC2 JC1 CBIC-2 Inhibitor inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.