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WST-8

Catalog No. T4018 CAS 193149-74-5

Synonyms: WST8

WST-8 is a water-soluble tetrazolium dye, the sensitivity of the WST-8(WST8)-based assay is higher than the conventional MTS-based assay.

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WST-8, CAS 193149-74-5

Pack Size	Availability	Price/USD
25 mg	In stock	$ 50.00
50 mg	In stock	$ 72.00
100 mg	In stock	$ 109.00
200 mg	In stock	$ 197.00

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Purity: 100%

Purity: 99.93%

Purity: 99.79%

Purity: 99.78%

Purity: 99.72%

Purity: 99.68%

Purity: 99.51%

Purity: 98.08%

Purity: 97.22%

Purity: 97.18%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

WST-8 is a water-soluble tetrazolium dye, the sensitivity of the WST-8(WST8)-based assay is higher than the conventional MTS-based assay.

In vivo

NS1209 could be safely administered to rats undergoing electrically induced SE at doses up to 50 mg/kg followed by intravenous infusion of 5 mg/kg for up to 24h. NS1209 administered as a bolus dose of 10-50 mg/kg (i.p. or i.v.) followed by infusion of 4 or 5 mg/kg h (i.v.) for 2-24h effectively discontinued electrically induced SE in all animals within 30-60 min, and there was no recurrence of SE after a 24-h infusion. Kainate-induced SE was similarly blocked by 10 or 30 mg/kg NS1209 (i.v.). To compare the efficacy and neuroprotective effects of NS1209 with those of diazepam (DZP), one group of rats received DZP (20 mg/kg, i.p. and another dose of 10 mg/kg 6h later). By using the administration protocols described, the anticonvulsant effect of NS1209 was faster and more complete than that of DZP. NS1209 treatment (20 mg/kg bolus followed by 5 mg/kg h infusion for 24 h) was neuroprotective against SE-induced hippocampal neurodegeneration, but to a lesser extent than DZP.

Synonyms	WST8
Molecular Weight	601.48
Formula	C20H14N6NaO11S2+
CAS No.	193149-74-5

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 50 mg/mL (83.13 mM), Sonication is recommended.

DMSO: 10 mg/mL (16.63 mM), Sonication is recommended.

References and Literature

1. Tiwari K, et al. A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line. Pharm Biol. 2015 Jun;53(6):849-54.

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

WST-8 193149-74-5 Others WST8 inhibit Inhibitor WST 8 inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.