BAMEAO16B is a lipid nanoparticle that incorporates disulfide bonds for efficient delivery of Cas9 mRNA and sgRNA into cells. In the intracellular environment, BAMEAO16B responds to reduction by releasing RNA, facilitating genome editing. This compound is valuable for gene editing research purposes.

Treatment with BAMEA-O16B/Cas9 mRNA/sgHPV18 significantly inhibits the growth of HeLa cells, showcasing superior effectiveness over the application of scrambled sgRNA and Cas9 mRNA delivery. BAMEA-O16B demonstrates notable efficiencies in RNA delivery, mRNA encapsulation, and GFP knockout, indicating its effectiveness in directing Cas9 mRNA to regulate endogenous gene expression accurately. Additionally, cells treated with BAMEA-O16B/RNA exhibit enhanced endosome escape, outperforming those treated with BAMEA-O16/RNA. Furthermore, nanoparticles composed of BAMEA-O16B/RFP mRNA achieve efficient RFP expression in HeLa cells, underscoring the compound’s proficiency in genetic manipulation and expression[1].

The BAMEA-O16B/Cas9 mRNA/sgRNA (I.v.) nanoparticle significantly reduces the serum proprotein convertase subtilisin/kexin type 9 (PCSK9) levels in mice to 20% of those observed in untreated counterparts. Similarly, administration of BAMEA-O16B/Cas9 mRNA/sgPCSK9 nanoparticles lowers the PCSK9 concentration in mouse serum to 20% compared to levels following DPBS injection or treatment with BAMEA-O16B/Cas9 mRNA/scramblesgRNA nanoparticles[1].