COX-2/PI3K-IN-2 (compound 5f) possesses anti-inflammatory and anti-cancer properties, functioning as a selective inhibitor of COX-2 (Ki = 3.02 nM) and a potent inhibitor of PI3K (IC50 = 2.78 nM) [1].

COX-2/PI3K-IN-2 (compound 5f), at concentrations of 50-500 μg/mL over 72 hours, demonstrates significant antitumor effects on Breast cancer (MCF-7) cells with 40% cell death at 1.65 μM. At 10-150 μg/mL, it also exhibits antioxidant capabilities, with H2O2 scavenging comparable to ascorbic acid and stronger lysozyme inhibition (IC50=3.25 nM), highlighting its dual functional capacity in anticancer activity and antioxidant properties.