HM03 trihydrochloride, a potent and selective inhibitor of HSPA5 (also known as Bip or Grp78), exhibits anticancer activity.

HM03 trihydrochloride, at concentrations ranging from 0.1-50 μM and applied over 72 hours, demonstrates significant inhibitory activity, achieving over 50% inhibition at a 25 μM concentration in HCT116 cells[1]. It preferentially interacts with HSPA5 and HSPA9, forming more binding interactions compared to other HSP70 proteins. This compound has shown promising results in assays evaluating cancer cell viability and tumor inhibition[1].