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Pack Size | Price | Availability | Quantity |
---|---|---|---|
10 mg | $100 | Backorder | |
25 mg | $180 | Backorder | |
50 mg | $310 | Backorder | |
100 mg | $510 | Backorder |
Description | Hydrastinine hydrochloride is a semisynthetic alkaloid from the hydrolysis of the alkaloid Hydrastine, used as a haemostatic drug. |
Kinase Assay | Cyclic AMP-dependent protein kinase activity is assayed in a reaction mixture containing, in a final volume of 0.2 mL, 50 mM Tris-HCl (pH 7.0), 10 mM magnesium acetate, 2 mM EGTA, 1 μM cyclic AMP or absence of cyclic AMP, 3.3 to 20 μM [r-32P] ATP (4×105 c.p.m.), 0.5 μg of the enzyme, 100 μg of histone H2B and compound. The mixture is incubated at 30°C for 5 min. The reaction is terminated by adding 1mL of ice-cold 20% trichloroacetic acid after adding 500 μg of bovine serum albumin as a carrier protein. The sample is centrifuged at 3000 r.p.m. for 15min, the pellet is resuspended in ice-cold 10% trichloro-acetic acid solution and the centrifugation-resuspension cycle is repeated three times. The final pellet is dissolved in 1 mL of 1 N NaOH and radioactivity is measured with a liquid scintillation counter[1]. |
Molecular Weight | 225.67 |
Formula | C11H12ClNO2 |
Cas No. | 4884-68-8 |
Storage | |||||||||||||||||||||
Solubility Information | H2O: 10 mM | ||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||
H2O
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