L-690330 hydrate is a competitive inositol monophosphatase (IMPase) inhibitor with Ki values for human and bovine frontal cortex IMPase at 0.30 and 0.42 μM, respectively, and Ki values of 0.27 and 0.19 μM for recombinant human and bovine IMPase, respectively [1].

L-690330 hydrate, at a concentration of 50 μM and applied for 1 hour, stimulates P-AMPK and autophagy, enhancing the expression of LC3-I/II and p-AMPK in HEK293 cells [2].

Administering L-690330 hydrate via intracerebroventricular injection (0.1 μmol) to eight-week-old male ICR mice did not impact their motor activity and coordination, as observed in the beam walking test. However, there was a noted reduction in the time spent in light during the dark/light test [3]. This dosage and method of administration did not adversely affect the well-being of the mice.