Methyl-β-cyclodextrin (Methyl-beta-cyclodextrin) is a macrocyclic compound used as a solubilizer for hydrophobic compounds in biological experiments. Methyl-β-cyclodextrin is also a lipid raft inhibitor with cholesterol-lowering activity and potential antitumor activity.

METHODS: Five primary exudative lymphoma (PEL) cell lines, BCBL-1, BC-1, BC-3, TY-1, and GTO, were treated with Methyl-β-cyclodextrin (0-10 mM) for 24 h, and cell viability was measured by MTT assay.
RESULTS: Methyl-β-cyclodextrin dose-dependently inhibited the growth of PEL cells with IC50 between 3.33-4.23 mM. [1]
METHODS: Chicken hepatocellular carcinoma cells, LMH, were treated with Methyl-β-cyclodextrin (10 mM) for 1 h, then cholesterol (50 µg/mL) was added at different time points before, during, and after infection with FAdV-4, and the expression levels of the target proteins were detected by Western Blot.
RESULTS: When LMH cells were pretreated with increasing concentrations of Methyl-β-cyclodextrin prior to infection, penton protein levels decreased in a dose-dependent manner. [2]

METHODS: To test the antitumor activity in vivo, Methyl-β-cyclodextrin (500 mg/kg) was intraperitoneally injected into NRJ mice bearing PEL tumor BCBL-1 once a day for twenty-one days.
RESULTS: Methyl-β-cyclodextrin significantly inhibited the growth and invasion of PEL cells without significant adverse effects. [1]
METHODS: To investigate the modulatory effects on collagen, Methyl-β-cyclodextrin (1.25-5.0 mg/mouse) was intradermally injected into the SKH1 mouse twice a week for two months.
RESULTS: Methyl-β-cyclodextrin showed potent COL I up-regulatory activity resulting in increased skin thickness. [3]

PEL cells are incubated in triplicate in a 96-well microculture plate in the presence of different concentrations of methyl-β-cyclodextrin (0-10 mM) in a final volume of 0.1 mL for 24 h at 37°C. Subsequently, MTT (0.5 mg/mL final concentration) is added to each well. After 3 h of additional incubation, 100 μL of a 0.04 N HCl is added to dissolve the crystals. Absorption values at 570 nm are determined.

PEL cells are incubated in triplicate in a 96-well microculture plate in the presence of different concentrations of methyl-β-cyclodextrin (0-10 mM) in a final volume of 0.1 mL for 24 h at 37°C. Subsequently, MTT (0.5 mg/mL final concentration) is added to each well. After 3 h of additional incubation, 100 μL of a 0.04 N HCl is added to dissolve the crystals. Absorption values at 570 nm are determined[1].