NMac1 (Nm23 activator 1) is a natural compound, cassumunene, derived from Zingiber cassumunar Roxb. and traditionally used as an anti-inflammatory agent in East Asia. NMac1 exhibits anti-metastatic potential by directly interacting with the C-terminal of Nm23-H1, activating its NDP kinase (NDPK) enzymatic activity in vitro. NMac1 inhibits breast cancer metastasis in vivo.

NDPK:10.7 μM (EC50)

NMac1 is a potent activator of NDPK protein, activating NDPK activity of recombinant Nm23-H1 in a dose-dependent manner with an EC50 of 10.7 μM.
The morphological changes observed in MDA-MB-231 cells after treatment with NMac1 (25 μM) included substantial changes in the original height mesenchymal type (with a large number of folds) to a non-metastatic form with reduced folds and increased cell coverage.
NMac1 significantly reduced invasion and migration of MDA-MB-231 cells in a dose-dependent manner without affecting their proliferation. [1]

In NOD/SCID mice injected in situ with MDA-MB-231-LUS-D3H2LN cells, treatment with NMac1 (10 mg/Kg, 3 weeks) significantly inhibited breast cancer metastasis without affecting primary tumor size. [1]