Peroxidase, found in the roots of horseradish, actively involves in oxidizing reactive oxygen species, innate immunity, hormone biosynthesis, and pathogenesis of several diseases.

Peroxidases are enzymes found across nearly all living organisms, existing as monomers, dimers, or tetramers, and are encoded by genes located on various chromosomes. They display organ-specific, tissue-specific, cell-specific, and sub-cellular distribution patterns, fulfilling distinct functions. This vast family of isoenzymes, typically containing heme and varying in molecular weight from 35-100 Kd, includes mammalian peroxidases, which are significantly larger (576-738 amino acids) compared to those found in plants[1].