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PIPES

PIPES
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Purity:99.02%
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PIPES

Catalog No. T40645Cas No. 5625-37-6
PIPES (1,4-Piperazinediethanesulfonic acid) serves as a crucial component of the buffering agent in biochemical applications, known as PIPES buffer.
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5 g$29In Stock
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Product Introduction

Bioactivity
Description
PIPES (1,4-Piperazinediethanesulfonic acid) serves as a crucial component of the buffering agent in biochemical applications, known as PIPES buffer.
In vitro
To prepare the PIPES buffer, dissolve 173 g of 1,4-piperazinediethanesulfonic acid in 1 L of deionized water. Adjust the pH of the solution to 6.8 using sodium hydroxide pellets. This buffer is efficacious in mitigating lipid loss and artifacts induced by glutaraldehyde fixation [1].
Alias1,4-Piperazinediethanesulfonic acid, 1,4-piperazine bis(ethanesulfonic acid), 1,4-Piperazinebis(ethanesulfonic acid), PIPES
Chemical Properties
Molecular Weight302.37
FormulaC8H18N2O6S2
Cas No.5625-37-6
Storage & Solubility Information
Storage Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
DMSO: < 1mg/ml (insoluble)
H2O: 5 mg/mL (16.5 mM)
Solution Preparation Table
H2O
1mg5mg10mg50mg
20 mM0.1654 mL0.8268 mL1.6536 mL8.2680 mL
50 mM0.0661 mL0.3307 mL0.6614 mL3.3072 mL
100 mM0.0331 mL0.1654 mL0.3307 mL1.6536 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
1 Enter information below:
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2 Enter the in vivo formulation:
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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc