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PIPES

Name: PIPES
Brand: TargetMol
SKU: T40645
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No. T40645Cas No. 5625-37-6

Alias PIPES, 1,4-Piperazinediethanesulfonic acid, 1,4-Piperazinebis(ethanesulfonic acid), 1,4-piperazine bis(ethanesulfonic acid)

PIPES (1,4-Piperazinediethanesulfonic acid) is an essential component of buffering agents used in biochemical applications, commonly referred to as PIPES buffer.

PIPES

Purity: 99%

Catalog No. T40645Alias PIPES, 1,4-Piperazinediethanesulfonic acid, 1,4-Piperazinebis(ethanesulfonic acid), 1,4-piperazine bis(ethanesulfonic acid)Cas No. 5625-37-6

PIPES (1,4-Piperazinediethanesulfonic acid) is an essential component of buffering agents used in biochemical applications, commonly referred to as PIPES buffer.

Pack Size	Price	Availability	Quantity
5 g	$29	In Stock

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Purity:99%

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COA HNMR LCMS

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Product Introduction

Bioactivity

Description	PIPES (1,4-Piperazinediethanesulfonic acid) is an essential component of buffering agents used in biochemical applications, commonly referred to as PIPES buffer.
In vitro	To prepare the PIPES buffer, dissolve 173 g of 1,4-piperazinediethanesulfonic acid in 1 L of deionized water. Adjust the pH of the solution to 6.8 using sodium hydroxide pellets. This buffer is efficacious in mitigating lipid loss and artifacts induced by glutaraldehyde fixation [1].
Alias	PIPES, 1,4-Piperazinediethanesulfonic acid, 1,4-Piperazinebis(ethanesulfonic acid), 1,4-piperazine bis(ethanesulfonic acid)

Chemical Properties

Molecular Weight	302.37
Formula	C₈H₁₈N₂O₆S₂
Cas No.	5625-37-6

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: < 1 mg/mL (insoluble）

H2O: 5 mg/mL (16.5 mM), when pH is adjusted to 7 with NaOH. Sonication is recommended.

Solution Preparation Table

H2O

	1mg	5mg	10mg	50mg
1 mM	3.3072 mL	16.5360 mL	33.0721 mL	165.3603 mL
5 mM	0.6614 mL	3.3072 mL	6.6144 mL	33.0721 mL
10 mM	0.3307 mL	1.6536 mL	3.3072 mL	16.5360 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc