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PIPES (1,4-Piperazinediethanesulfonic acid) is an essential component of buffering agents used in biochemical applications, commonly referred to as PIPES buffer.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
5 g | $29 | In Stock |
Description | PIPES (1,4-Piperazinediethanesulfonic acid) is an essential component of buffering agents used in biochemical applications, commonly referred to as PIPES buffer. |
In vitro | To prepare the PIPES buffer, dissolve 173 g of 1,4-piperazinediethanesulfonic acid in 1 L of deionized water. Adjust the pH of the solution to 6.8 using sodium hydroxide pellets. This buffer is efficacious in mitigating lipid loss and artifacts induced by glutaraldehyde fixation [1]. |
Alias | PIPES, 1,4-Piperazinediethanesulfonic acid, 1,4-Piperazinebis(ethanesulfonic acid), 1,4-piperazine bis(ethanesulfonic acid) |
Molecular Weight | 302.37 |
Formula | C8H18N2O6S2 |
Cas No. | 5625-37-6 |
Smiles | OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 |
Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
Solubility Information | DMSO: < 1 mg/mL (insoluble) H2O: < 1 mg/mL (insoluble), when pH is adjusted to 7 with NaOH. Sonication is recommended. |
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