Propantheline is an orally active antagonist of muscarinic acetylcholine receptors (mAChRs), commonly employed to investigate smooth muscle dysfunction, hyperhidrosis, smooth muscle spasms or cramps in the stomach, intestines, or bladder, as well as involuntary urination [1] [2] [3].

Propantheline at concentrations ranging from 10 μM to 1 mM reduces the responsiveness of urinary bladder smooth muscle to Acetylcholine [1].

Propantheline, administered orally at doses ranging from 10-300 mg/kg, effectively reduced both fecal pellet count and the incidence of diarrhea in rats suffering from restraint stress-induced bowel dysfunction [3]. The compound demonstrated a dose-dependent efficacy, achieving an ED50 value of 41 mg/kg for decreasing fecal pellet count and an ED50 value of 64 mg/kg for reducing diarrhea incidence.