Seocalcitol is an analog of vitamin D, binds vitamin D receptor protein from human osteosarcoma MG-63 cells(Kd : 0.27 nM).

Vitamin D receptor:(kd)0.27 nM

Seocalcitol is a stimulators of osteoclast recruitment in murine bone marrow cultures(EC50 at 0.1 nM). Seocalcitol stimulates bone resorption(EC50 of 0.03 nM)[1]. Seocalcitol elicites a dose-dependent induction of 24-hydroxylase mRNA in the kidney (EC50=0.4±0.13). In the kidney, Kd values for Seocalcitol is 0.48±0.04 nM. However, in the intestine, the Kd for Seocalcitol is 1.43±0.19 nM)[2]. Seocalcitol (0.1-10 nM) induces cell differentiation in a dosedependent manner. A higher differentiating activity is observed for 1 nM Seocalcitol than for 1 nM VD3.

Seocalcitol, a synthetic vitamin D analog, demonstrates lower hypercalcemic activity compared to 1,25(OH)2VD3. Its long-term intraperitoneal (IP) administration at 0.5 μg/kg body weight bi-daily significantly inhibits hepatocellular carcinoma (HCC) development in C3H/Sy mice[4]. Furthermore, when administered intraperitoneally to postnatal rats from days 4 to 12 at dosages of 0.38 or 1.25 μg/kg body weight per day, only the higher dosage (1.25 μg/kg) significantly attenuates weight gain, either as a standalone treatment or when combined with Dexamethasone, all-trans retinoic acid (RA), or retinoic acid[5].