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TMB dihydrochloride

Catalog No. T19069Cas No. 64285-73-0
Alias Sure Blue TMB dihydrochloride, BM blue dihydrochloride, 3,3',5,5'-Tetramethylbenzidine dihydrochloride

TMB dihydrochloride (Sure Blue TMB dihydrochloride) is an enzyme-linked immunosorbent assays (ELISA) and an excellent colorimetric substrate for the detection of horseradish peroxidase (HRP)-labeled probes used in staining procedures immunohistochemistry.

TMB dihydrochloride

TMB dihydrochloride

Purity: 99.98%
Catalog No. T19069Alias Sure Blue TMB dihydrochloride, BM blue dihydrochloride, 3,3',5,5'-Tetramethylbenzidine dihydrochlorideCas No. 64285-73-0
TMB dihydrochloride (Sure Blue TMB dihydrochloride) is an enzyme-linked immunosorbent assays (ELISA) and an excellent colorimetric substrate for the detection of horseradish peroxidase (HRP)-labeled probes used in staining procedures immunohistochemistry.
Pack SizePriceAvailabilityQuantity
1 g$29In Stock
1 mL x 10 mM (in DMSO)$29In Stock
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Purity:99.98%
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Product Introduction

Bioactivity
Description
TMB dihydrochloride (Sure Blue TMB dihydrochloride) is an enzyme-linked immunosorbent assays (ELISA) and an excellent colorimetric substrate for the detection of horseradish peroxidase (HRP)-labeled probes used in staining procedures immunohistochemistry.
AliasSure Blue TMB dihydrochloride, BM blue dihydrochloride, 3,3',5,5'-Tetramethylbenzidine dihydrochloride
Chemical Properties
Molecular Weight313.27
FormulaC16H22Cl2N2
Cas No.64285-73-0
Storage & Solubility Information
Storagekeep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 55 mg/mL (175.57 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM3.1921 mL15.9607 mL31.9213 mL159.6067 mL
5 mM0.6384 mL3.1921 mL6.3843 mL31.9213 mL
10 mM0.3192 mL1.5961 mL3.1921 mL15.9607 mL
20 mM0.1596 mL0.7980 mL1.5961 mL7.9803 mL
50 mM0.0638 mL0.3192 mL0.6384 mL3.1921 mL
100 mM0.0319 mL0.1596 mL0.3192 mL1.5961 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
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