The recognized V5 epitope represents 95GKPIPNPLLGLDST108 of RNA polymerase α subunit of simian parain uenza virus type 5. This short peptide sequence was chosen because high-af nity antibodies can be reliably produced in many different species1.

A 14-amino-acid V5 epitope derived from simian parain?uenza virus type 5 was inserted into the C terminus of the capsid protein (before the stop codon ofORF2) to construct a recombinant marker virus. We demonstrated that the V5 epitope was displayed on the surface of the capsid protein. Furthermore, the recombinant marker virus behaved similarly to the parental virus in vitro and in mice and could be differentiated from the parental virus via polymerase chain reaction (PCR) and serological methods [2][3].

A V5/His-epitope tag was added to the amino terminus of each coding sequence to distinguish the plasmid-encoded protein from endogenous LRRK2. Plasmids were transiently transfected into COS7 cells, and cell lysates were analyzed by SDS-PAGE and Western blotting with antibodies speci?c for the V5 epitope tag or LRRK24.