A33 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-01794

A33 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.4 kDa and the accession number is A0A0R4J209.

A33 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-01794

A33 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.4 kDa and the accession number is A0A0R4J209.

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50 μg	$357	7-10 days

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Product Information

Biological Activity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	A33 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25.4 kDa and the accession number is A0A0R4J209.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q9JKA5
Synonyms	glycoprotein A33 (transmembrane),BB116197,2210401D16Rik,2010310L10Rik
Construction	A DNA sequence encoding the mouse GPA33 (NP_067623.1) extracellular domain (Met 1-Ile 235) was expressed, with a polyhistidine tag at the C-terminus. Predicted N terminal: Leu 22
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	25.4 kDa (predicted); 35-40 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I membrane protein that is expressed in the normal gastrointestinal epithelium and 95% of colon cancers. GPA33 contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation. GPA33 has become a promising target of immunologic therapy strategies, but its biologic function and potential role in tumorigenesis are unknown. EpCAM protein and GPA33 mRNA expressions are specific and sensitive markers of Barrett's metaplasia (BM). GPA33 may also play a role in cell-cell recognition and signaling.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy