1. Immobilized Human GPA33, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. 2. Anti-GPA33 Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human GPA33, His Tag with an affinity constant of 0.69 nM as determined in SPR assay.

GPA33/A33 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 26.5 kDa and the accession number is Q99795-1.

Human

HEK293 Cells

MGC129987,MGC129986,GPA33,Glycoprotein A33,A33

Ile22-Val235

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.