A33 Protein, Human, Recombinant (His)

Catalog No. TMPY-02012

A33 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25 kDa and the accession number is Q99795-1.

A33 Protein, Human, Recombinant (His)

Catalog No. TMPY-02012

A33 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25 kDa and the accession number is Q99795-1.

Pack Size	Price	Availability	Quantity
50 μg	$357	7-10 days
500 μg	$1,950	7-10 days

Bulk & Custom

Add to Cart

Questions

Resource Download

User Guide of Recombinant Proteins

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Product Information

Biological Activity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	A33 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 25 kDa and the accession number is Q99795-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	Q99795-1
Synonyms	glycoprotein A33 (transmembrane),A33
Construction	A DNA sequence encoding the extracellular domain of human GPA33 (Q99795-1) (Met 1-Val 235) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Ile 22
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	25 kDa (predicted); 35-40 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I membrane protein that is expressed in the normal gastrointestinal epithelium and 95% of colon cancers. GPA33 contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation. GPA33 has become a promising target of immunologic therapy strategies, but its biologic function and potential role in tumorigenesis are unknown. EpCAM protein and GPA33 mRNA expressions are specific and sensitive markers of Barrett's metaplasia (BM). GPA33 may also play a role in cell-cell recognition and signaling.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy