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B7-1 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P33681-1.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $588 | Backorder | |
100 μg | $1,380 | Backorder |
Biological Activity | Immobilized CTLA-4 Protein, Human, Recombinant (hFc) (Cat#TMPY-05002) at 2 μg/mL (100 μl/well) can bind B7-1 Protein, Human, Recombinant (hFc & Avi), Biotinylated (Cat#TMPY-07030), the EC50 is 0.7-2.1 ng/mL. |
Description | B7-1 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is P33681-1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-hFc-Avi |
Accession Number | P33681-1 |
Synonyms | LAB7,CD28LG1,CD28LG,BB1,B7-1,B7.1,B7 |
Construction | A DNA sequence encoding the Human CD80 (NP_005182.1) (Met1-Asn242) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
Protein Purity | ≥ 90% as determined by SDS-PAGE. ≥ 95% as determined by SEC-HPLC. |
Molecular Weight | 52.4 kDa (predicted); 73.6 kDa (reducing contition, due to glycosylation) |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
Reconstitution | Please refer to the lot-specific COA. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: IHC AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
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