BAFF/TNFSF13B Protein, Human, Recombinant (monomeric hFc, Flag)

Catalog No. TMPK-00175

B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. BAFF/TNFSF13B Protein, Human, Recombinant (monomeric hFc, Flag) is expressed in HEK293 mammalian cells with N-monomeric hFc and N-Flag tag. The predicted molecular weight is 74.9 kDa and the accession number is Q9Y275-1.

BAFF/TNFSF13B Protein, Human, Recombinant (monomeric hFc, Flag)

Catalog No. TMPK-00175

Pack Size	Price	Availability
100 μg	$511	7-10 days
500 μg	$2,040	7-10 days
1 mg	$3,410	7-10 days

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Product Information

Biological Activity	1. Immobilized Human BAFF (Trimer) , hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human BAFFR, His Tag with the EC50 of 1.45μg/ml determined by ELISA (QC Test). 2. Human BAFFR, His Tag captured on CM5 Chip via anti-his antibody can bind Human BAFF (Trimer) , hFc-Flag Tag with an affinity constant of 1.21 nM as determined in SPR assay.
Description	B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. BAFF/TNFSF13B Protein, Human, Recombinant (monomeric hFc, Flag) is expressed in HEK293 mammalian cells with N-monomeric hFc and N-Flag tag. The predicted molecular weight is 74.9 kDa and the accession number is Q9Y275-1.
Species	Human
Expression System	HEK293 Cells
Tag	N-monomeric hFc, N-Flag
Accession Number	Q9Y275-1
Synonyms	ZTNF4,TNFSF20,TNFSF13B,THANK,TALL1δ4 BAFF,TALL-1δ BAFF,TALL1Delta4 BAFF,TALL-1delta BAFF,TALL-1,TALL1,DTL,CD257,BLyS,BAFF
Construction	Thr141-Leu285
Protein Purity	> 95% as determined by Tris-Bis PAGE> 94% as determined by HPLC
Molecular Weight	74.9 kDa (predicted) same as Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.