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BAFF/TNFSF13B Trimer Protein, Human, Recombinant (His & Flag)
Catalog No. TMPK-00176
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. BAFF/TNFSF13B Trimer Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 52.4 kDa and the accession number is Q9Y275-1.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose. | Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 100 μg | $511 | In Stock | |
| 500 μg | $2,040 | 7-10 days | |
| 1 mg | $3,410 | 7-10 days |
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Biological Description
| Biological Information | 1. Immobilized Human BAFF Trimer, His Tag at 2 μg/mL (100 μL/well). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 56.9 ng/mL determined by ELISA._x000D_
2. Immobilized Human BAFF Trimer, His Tag at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Human TACI, hFc Tag with the EC50 of 3.5 ng/mL determined by ELISA._x000D_
3. Human BAFF Trimer, His Tag captured on CM5 Chip via Anti-His Antibody can bind Mouse BCMA, hFc Tag with an affinity constant of 6.59 nM as determined in SPR assay (Biacore T200). |
| Description | B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. BAFF/TNFSF13B Trimer Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 52.4 kDa and the accession number is Q9Y275-1. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | N-His-Flag |
| Accession Number | Q9Y275-1 |
| Synonyms | ZTNF4,TNFSF20,TNFSF13B,THANK,TALL1δ4 BAFF,TALL-1δ BAFF,TALL1Delta4 BAFF,TALL-1delta BAFF,TALL-1,TALL1,DTL,CD257,BLyS,BAFF |
| Construction | Thr141-Leu285 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Molecular Weight | 52.4 kDa (predicted). Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result. |
| Endotoxin | < 1 EU/μg by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, Lyophilized powders are shipping with blue ice. |
| Research Background | B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R. |
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