BDCA-2 Protein, Cynomolgus, Recombinant (His)

Catalog No. TMPK-00505

BDCA-2 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with N-His tag. The predicted molecular weight is 20.55 kDa and the accession number is A0A2K5UWP4.

BDCA-2 Protein, Cynomolgus, Recombinant (His)

Catalog No. TMPK-00505

BDCA-2 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with N-His tag. The predicted molecular weight is 20.55 kDa and the accession number is A0A2K5UWP4.

Pack Size	Price	Availability
100 μg	$487	7-10 days
500 μg	$1,950	7-10 days
1 mg	$3,250	7-10 days

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Product Information

Biological Activity	Immobilized Cynomolgus BDCA-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.
Description	BDCA-2 Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with N-His tag. The predicted molecular weight is 20.55 kDa and the accession number is A0A2K5UWP4.
Species	Cynomolgus
Expression System	HEK293 Cells
Tag	N-His
Accession Number	A0A2K5UWP4
Synonyms	HECL,DLEC,CLECSF7,CLECSF11,CLEC4C,CD303,BDCA-2,BDCA2
Construction	Tyr48-Ile212
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	20.55 kDa (predicted). Due to glycosylation, the protein migrates to 28-38 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 300 mM L-arginine (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling.