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BDCA-2 Protein, Human, Recombinant (His & Avi), Biotinylated

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BDCA-2 Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPK-01118

BDCA-2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 22.7 kDa and the accession number is Q8WTT0-1.

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Pack Size	Price	Availability
100 μg	$814	In Stock
500 μg	$3,250	7-10 days
1 mg	$5,440	7-10 days

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Biological Description

Biological Information	Immobilized Biotinylated Human BDCA-2, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 3.5ng/ml determined by ELISA.
Description	BDCA-2 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 22.7 kDa and the accession number is Q8WTT0-1.
Species	Human
Expression System	HEK293 Cells
Tag	N-His-Avi
Accession Number	Q8WTT0-1
Synonyms	CLEC4C,BDCA-2,CLECSF7,CLECSF11,CD303,BDCA2,HECL,DLEC
Construction	Phe46-Ile213
Protein Purity	> 95% as determined by Tris-Bis PAGE
Molecular Weight	22.7 kDa (predicted). Due to glycosylation, the protein migrates to 33-40 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Supplied as 0.22 μm filtered solution in 20 mM Tris, 300 mM NaCl, 200 mM L-arginine (pH 8.5).
Stability & Storage	It is recommended to store the product under sterile conditions at -70°C or lower. Samples are stable for up to 12 months at -80°C. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	BDCA-2, BDCA-3, and BDCA-4. In blood, BDCA-2 and BDCA-4 are expressed on CD11c(-) CD123(bright) plasmacytoid dendritic cells, whereas BDCA-3 is expressed on small population of CD11c() CD123(-) dendritic cells. All three Ags are not detectable on a third blood dendritic cell population, which is CD1c() CD11c(bright) CD123(dim), or on any other cells in blood.BDCA-2 is completely down-regulated on plasmacytoid CD11c(-) CD123(bright) dendritic cells, BDCA-2 is rapidly internalized at 37 degrees C after mAb labeling.