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Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $814 | 7-10 days | |
500 μg | $3,250 | 7-10 days | |
1 mg | $5,440 | 7-10 days |
Biological Information | Immobilized Anti-CD161 Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CD161, His Tag with the EC50 of 41.8ng/ml determined by ELISA. |
Description | CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment. CD161 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 21.4 kDa and the accession number is Q12918-1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-His-Avi |
Accession Number | Q12918-1 |
Synonyms | NKRP1,HNKR-P1a,NKRP1A,CD161,NKR-P1A,Ly59,NKRP1ANKR,NKR,NKR-P1,KLRB1,CLEC5B |
Construction | Gln67-Ser225 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | 21.4 kDa (predicted). Due to glycosylation, the protein migrates to 41-45 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | CD161 (NKRP1) is a lectin-like receptor present on NK cells and rare T-cell subsets. We have observed CD161 expression in some cases of T-cell prolymphocytic leukemia (T-PLL) and found it to be useful in follow-up and detection of disease after treatment. |
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