CD1D Protein, Mouse, Recombinant (His)

Catalog No. TMPY-01397

CD1D Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.7 kDa and the accession number is A0A0R4J090.

CD1D Protein, Mouse, Recombinant (His)

Catalog No. TMPY-01397

CD1D Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.7 kDa and the accession number is A0A0R4J090.

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100 μg	$600	7-10 days

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Product Information

Biological Activity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	CD1D Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 33.7 kDa and the accession number is A0A0R4J090.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P11609
Synonyms	Ly-38,CD1d molecule,Cd1d,Cd1a,CD1.1,AI747460
Construction	A DNA sequence encoding the extracellular domain of mouse CD1D (NP_031665.2) (Met 1-Gly 305) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Gln 22
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	33.7 kDa (predicted); 45-50 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 1 (CD1) is a member of the CD system. It's a family of glycoproteins expressed on the surface of various human antigen-presenting cells which are implicated in the presentation of lipid antigens to T-cells. Due to the different lipid anchoring, the CD1 family is classified into two groups: group1 (CD1a-c) and group2 (CD1d). CD1d with lipid antigens activate NK T-cells which rapidly produce Th1 and Th2 cytokines after been activated.