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Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $462 | 7-10 days | |
100 μg | $1,070 | 7-10 days |
Biological Information | 1. Loaded Biotinylated Human FcγRIIA / CD32a recombinant protein (His & Avi Tag) on SA Biosensor, can bind Rituximab (IgG1) with an affinity constant of 0.62 μM as determined in a BLI assay.
2. The purity of Human CD32a / FCGR2A Protein (167 Arg, His & AVI Tag), Biotinylated is more than 95% and the molecular weight of this protein is around 22-34 kDa verified by SEC-MALS. |
Description | CD32A Protein, Human, Recombinant (167 Arg, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 23.7 kDa and the accession number is P12318-1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | His, Avi |
Accession Number | P12318-1 |
Synonyms | FcGR,CD32,IGFR2,Fc fragment of IgG receptor IIa,CDw32,FCGR2,CD32A,FCGR2A1,Fc γ RIIA,FCG2,Fc gamma RIIA |
Construction | The extracellular domain (Met1-Met210) of Human CD32a (P12318-1, with Natural variant His 167 Arg) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC |
Molecular Weight | 23.7 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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