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Fc gamma RI/CD64 Protein, Human, Recombinant (His & Avi), Biotinylated

Fc gamma RI/CD64 Protein, Human, Recombinant (His & Avi), Biotinylated
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Fc gamma RI/CD64 Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPK-00241
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. Fc gamma RI/CD64 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 33.5 kDa and the accession number is P12314-1.
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Pack SizePriceAvailabilityQuantity
100 μg$8147-10 days
500 μg$3,2507-10 days
1 mg$5,4407-10 days
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Biological Description

Biological Information
Biotinylated Human Fc gamma RI, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.91 nM as determined in SPR assay.
Description
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. Fc gamma RI/CD64 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 33.5 kDa and the accession number is P12314-1.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberP12314-1
Synonyms
FcERI,CD64,FLJ18345,IGFR1,Fc gamma RI,Fcgr,Fcr,Fc γ RI/CD64,CD64a,FCG1,FcRIA,Fc γ RI,FcgRI,FCE1A,FCGR1,Fc-gamma RIA,FCGR1A,FCRI,Fc-γ RIA
Construction
Gln16-Pro288
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight33.5 kDa (predicted). Due to glycosylation, the protein migrates to 55-68 kDa based on Tris-Bis PAGE result.
Endotoxin< 1 EU/μg by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.

Dose Conversion

You can also refer to dose conversion for different animals. More

Tech Support

Please read the User Guide of Recombinant Proteins for more specific information.

Keywords