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Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $296 | In Stock | |
100 μg | $690 | 7-10 days | |
200 μg | $1,180 | 7-10 days | |
500 μg | $2,380 | 7-10 days |
Biological Information | Loaded Biotinylated Human FcγRIIB/C / CD32b/c protein (His & Avi Tag) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 1.2 μM as determined in a BLI assay (QC tested). |
Description | CD32B/Fcgr2b Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 24 kDa and the accession number is P31994-2. |
Species | Human |
Expression System | HEK293 Cells |
Tag | His, Avi |
Accession Number | P31994-2 |
Synonyms | IGFR2,FCGR2A,FCGR21,FCGR2,FcGR,FCG2,Fc γ RIIB,Fc γ RII,Fc gamma RIIB,Fc gamma RII,Fc fragment of IgG, low affinity IIb, receptor (CD32),CDw32,CD32B,CD32A,CD32 |
Construction | A DNA sequence encoding the extracellular domain (Ala 46-Ile 224) of mature human CD32b (NP_001002274.1) protein was fused with a signal peptide at the N-terminus and a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Ala 46 |
Protein Purity | > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC |
Molecular Weight | 24 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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