1. Immobilized Rituximab (IgG1) at 1 μg/mL can bind Mouse FcγRI / CD64 recombinant protein, the EC50 is 16-35 ng/mL. 2. Immobilized Anti-CD3 antibody, mouse IgG2a at 2 μg/mL (100 μL/well) can bind Recombinant Mouse CD64 / FCGR1 Protein (His Tag) , the EC50 is 0.8-2.4 ng/mL(QC tested). 3.Loaded Mouse FcγRI / CD64 recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.12 µM as determined in a BLI assay. 4.Loaded Mouse FcγRI / CD64 recombinant protein on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 0.28 µM as determined in a BLI assay. 5.Captured  Mouse FcγRI / CD64 recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.12 µM as determined in an SPR assay. 6.Captured Mouse FcγRI / CD64 recombinant protein  on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.69 µM as determined in an SPR assay (Biacore T200). 7. The purity of Mouse CD64 / FCGR1 Protein (His Tag) is more than 95% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS. 8.Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a on ProA Biosensor, can bind Recombinant Mouse CD64 Protein, His Tag with an affinity constant of 3.46nM as determined in BLI assay(QC tested).

CD64 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 32 kDa and the accession number is P26151.

Mouse

HEK293 Cells

IGGHAFC,FcγRI,FcgammaRI,Fc receptor, IgG, high affinity I,CD64,AV092959,AI323638

A DNA sequence encoding the extracellular domain (Met 1-Pro 297) of mouse FCGR1 (NP_034316.1) precursor was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Glu 25

> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.