Measured by its binding ability in a functional ELISA. Immobilized human EPHA3-His at 10 μg/ml (100 μl/well) can bind human EphrinA5-Fc , The EC50 of human EphrinA5-Fc is 6.2-14.6 ng/ml.

EphA3 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.3 kDa and the accession number is A0A140VJJ0.

Human

HEK293 Cells

TYRO4,HEK4,HEK,ETK1,ETK,EPH receptor A3,EK4

A DNA sequence encoding the human EPHA3 (NP_005224.2) (Met1-Gln541) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Glu 21

> 95 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

EPHA3 gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 gene encodes a protein that binds ephrin-A ligands. EPHA3 is involved in the retinotectal mapping of neurons. It may also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy