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FGFR2 beta (IIIb) Domain Protein, Human, Recombinant (Avi), Biotinylated

Catalog No. TMPK-00399
FGFR2 beta (IIIb) Domain Protein, Human, Recombinant (Avi), Biotinylated is expressed in HEK293 mammalian cells with C-Avi tag. The predicted molecular weight is 15.49 kDa and the accession number is P21802-3.
Pack SizePriceAvailabilityQuantity
100 μg$8147-10 days
500 μg$3,2507-10 days
1 mg$5,4407-10 days
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Product Information

Biological Activity
Immobilized Biotinylated Human FGFR2 beta (IIIb) Domain, Avi Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-FGFR2 (IIIb) Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA.
Description
FGFR2 beta (IIIb) Domain Protein, Human, Recombinant (Avi), Biotinylated is expressed in HEK293 mammalian cells with C-Avi tag. The predicted molecular weight is 15.49 kDa and the accession number is P21802-3.
Species
Human
Expression System
HEK293 Cells
TagC-Avi
Accession NumberP21802-3
Synonyms
K-sam,KSAM,KGFR,Keratinocyte growth factor receptor,Fibroblast growth factor receptor 2,FGFR2 β (IIIb) Domain Protein,FGFR-2,CD332,BEK
Construction
Pro253-Glu378
Protein Purity
> 95% as determined by Tris-Bis PAGE; ; > 90% as determined by HPLC
Molecular Weight15.49 kDa (predicted). Due to glycosylation, the protein migrates to 32-50 kDa based on Tris-Bis PAGE result.
Endotoxin< 1 EU/μg by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform.

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