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Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $814 | 7-10 days | |
500 μg | $3,250 | 7-10 days | |
1 mg | $5,440 | 7-10 days |
Biological Information | Immobilized Anti-FGFR2 IIIb Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human FGFR2 beta (IIIb) , His Tag with the EC50 of 13.1ng/ml determined by ELISA. |
Description | FGFR2 beta (IIIb) Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.3 kDa and the accession number is P21802-3. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-His-Avi |
Accession Number | P21802-3 |
Synonyms | KGFR,FGFR-2,KSAM,CD332,BEK,FGFR2 β (IIIb),Keratinocyte growth factor receptor,K-sam,Fibroblast growth factor receptor 2 |
Construction | Arg152-Glu378 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | 28.3 kDa (predicted). Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform. |
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