IL-2 R alpha/CD25 Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00164

Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. IL-2 R alpha/CD25 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 24.6 kDa and the accession number is P01589.

IL-2 R alpha/CD25 Protein, Human, Recombinant (His & Avi)

Catalog No. TMPK-00164

Pack Size	Price	Availability
100 μg	$465	7-10 days
500 μg	$1,850	7-10 days
1 mg	$3,100	7-10 days

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Product Information

Biological Activity	1. Immobilized Human IL-2 R alpha, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA. 2. Human IL-2 R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human IL-2, No Tag with an affinity constant of 15.2nM as determined in SPR assay.
Description	Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells. IL-2 R alpha/CD25 Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 24.6 kDa and the accession number is P01589.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	P01589
Synonyms	TCGFR,p55,IL2RA,IL2R,IL-2 R α/CD25,IL-2 R α,IL-2 R alpha,IDDM10,CD25
Construction	Glu22-Cys213
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	24.6 kDa (predicted). Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.