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Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $801 | 7-10 days |
Biological Information | Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC . The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26 . The specific activity is >15 pmol/min/µg. |
Description | METAP2 Protein, Human, Recombinant (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 54.3 kDa and the accession number is A0A140VJE3. |
Species | Human |
Expression System | Baculovirus Insect Cells |
Tag | C-His |
Accession Number | A0A140VJE3 |
Synonyms | MAP2,MNPEP,p67eIF2,methionyl aminopeptidase 2,p67 |
Construction | The Human METAP2 (NP_006829.1) (Met1-Tyr478) was expressed with a polyhistidine tag at the C-terminus. |
Protein Purity | > 85 % as determined by SDS-PAGE. |
Molecular Weight | 54.3 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Supplied as sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a protein that belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis. |
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