Tested by Flow cytometric analysis of anti-CD33 CAR expression.

Siglec-3/CD33 Protein, Human, Recombinant, PE conjugated is expressed in HEK293 mammalian cells. The accession number is AAH28152.1.

Human

HEK293 Cells

Siglec-3,SIGLEC3,p67,CD33 molecule

Recombinant human CD33 (AAH28152.1, Met1-His259) are conjugated with PE under optimum conditions, the unreacted PE was removed.

This reagent is stable for 6 months when stored at 2°C-8°C. Protected from prolonged exposure to light. Do not freeze!

Myeloid cell surface antigen CD33 also known as Sialic acid binding Ig-like lectin 3, CD33 antigen or Siglec-3, is a member of the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. This Single-pass type I membrane protein contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain. CD33 /Siglec-3 is a putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. CD33 /Siglec-3 preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. CD33/Siglec-3 induces apoptosis in acute myeloid leukemia (in vitro). CD33/Siglec-3 can function as a sialic acid-dependent cell adhesion molecule and that binding can be modulated by endogenous sialoglycoconjugates when CD33 is expressed in a plasma membrane.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy