NECTIN4/Nectin 4 Protein, Human, Recombinant (hFc & AVI)

Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated

Catalog No. TMPY-06958

Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 62.65 kDa and the accession number is NP_112178.2.

Pack Size	Price	Availability	Quantity
20 μg	$600	7-10 days
100 μg	$1,390	7-10 days

Bulk & Custom

Add to Cart

QHow to reconstitute lyophilized recombinant proteins into a stock solution?

ACentrifuge the product at 10,000-12,000 rpm for 20-30 seconds before opening the vial to drive any protein that may be lodged in the cap or on the side of the tube to the bottom of the vial.In general, most proteins can be reconstituted by simply adding sterile distilled water. If other diluents are needed, they will be specified in the product's Certificate of Analysis (COA). It is recommended to prepare the stock solution with a concentration not lower than 100 μg/mL and aliquot for storage to avoid repeated freeze-thaw cycles.

QHow are recombinant protein products shipped?

AMost recombinant proteins are delivered as lyophilized powder, which are insensitive to temperature change and are stable at room temperature. They are typically shipped at room temperature or with blue ice. For liquid recombinant proteins, dry ice is generally used for shipping. It is normal if the product is arrived with a melted ice pack. The protein can still be used without any negative impact on its quality for a short period. If a specific protein is unstable at room temperature, appropriate cold chain shipping will be arranged to ensure product quality remains intact.

QWhy is the volume & weight of the product not as expected?

AThe lyophilized powder is obtained from the freeze-drying of protein solutions, and its weight is not equivalent to that of protein. The volume of powder is influenced by different factors, including the composition of the buffer prior to lyophilization, salt ion concentration, and the concentration of the product itself. Thus, it’s normal that the powder may appear plentiful or be skipped by the eye. Upon receiving the product, proceed with reconstitution, use, or aliquot storage as usual.

QWhy are carrier proteins needed in some recombinant protein solutions?

ACarrier proteins help improve the stability of proteins and prevent them from adhereing to the walls upon freeze and thaw. If proteins adhere to the tube, it will lead to a decrease in protein concentration in the solution which affects protein activity. For long term storage, carrier proteins, such as 0.1% Bovine Serum Albumin (BSA), 5% Human Serum Albumin (HAS), 10% Fetal Bovine Serum (FBS), or 5% trehalose, are used to reduce such loss. If the concentration of the recombinant protein is low, please add an appropriate amount of carrier protein to maintain protein activity.

QHow to store the recombinant protein products?

A1. It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, they should be used promptly. If they cannot be used up within one week, the product can be stored at -20°C to -80°C for at least 3 months. 2. Avoid multiple freeze-thaw cycles and store products in aliquots. When aliquoting and freezing, it is advisable to use a solution or medium with a certain concentration of carrier protein for reconstitution. The product concentration should not be less than 100 μg/mL, and the volume per vial should be no less than 20 μL.

Biological Activity	Immobilized Recombinant Human NECTIN4/Nectin 4 Protein (ECD, Fc & AVI Tag), Biotinylated on streptavidin precoated (2 μg/mL，100 μL/well) can bind Recombinant Human CD111 / Nectin-1 / PVRL1 Protein (His Tag), the EC50 is 40-120 ng/mL.
Description	Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 62.65 kDa and the accession number is NP_112178.2.
Species	Human
Expression System	HEK293 Cells
Tag	C-hFc-Avi
Accession Number	NP_112178.2
Synonyms	PVRL4,PRR4,nectin-4,LNIR,EDSS1
Construction	A DNA sequence encoding the Human NECTIN4 (NP_112178.2) (Met1-Ser349) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Gly 32
Protein Purity	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Molecular Weight	62.65 kDa (predicted); 76.3 kDa (reducing conditions)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.

Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated

Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated

Product Information

Dose Conversion

Calculator

Tech Support

Keywords