Immobilized Recombinant Human NECTIN4/Nectin 4 Protein (ECD, Fc & AVI Tag), Biotinylated on streptavidin precoated (2 μg/mL，100 μL/well) can bind Recombinant Human CD111 / Nectin-1 / PVRL1 Protein (His Tag), the EC50 is 40-120 ng/mL.

Nectin-4 Protein, Human, Recombinant (hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with hFc and Avi tag. The predicted molecular weight is 62.65 kDa and the accession number is NP_112178.2.

Human

HEK293 Cells

PVRL4,PRR4,nectin-4,LNIR,EDSS1

A DNA sequence encoding the Human NECTIN4 (NP_112178.2) (Met1-Ser349) was expressed with a c-terminal AVI tagged Fc region of human IgG1 at the C-terminus (Fc-AVI). The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Gly 32

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.