1. Loaded Biotinylated Human Nectin-4-His-Avi on SA Biosensor, can bind Anti-Human Nectin-4 mAb-mFc with an affinity constant of 1 <10^-3 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human Nectin-4-His-Avi at 2μg/ml (100 μl/well) can bind Anti-Human Nectin-4 mAb-mFc. The ED50 of Anti-Human Nectin-4 mAb-mFc is 12.28 ng/ml. (Regularly tested)

Nectin-4 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-6xHis-Avi tag. The predicted molecular weight is 40-50 KDa and the accession number is Q96NY8.

Human

HEK293 Cells

PVRL4,PRR4,Poliovirus receptor-related protein 4,Nectin-4,LNIR,Ig superfamily receptor LNIR

Gly32-Val351

Gly32-Val351

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Nectin-4 (PVRL4) is a type I transmembrane glycoprotein which belongs to the nectin family of Ig superfamily proteins. It contains two Ig-like C2-type domains and one Ig-like V-type domain. PVRL4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with nectin-1. It does not act as receptor for alpha-herpesvirus entry into cells. It is predominantly expressed in placenta, the embryo and breast carcinoma. But it is not detected in normal breast epithelium. The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder.