Nectin-4 Protein IgV Domain, Human, Recombinant (His & Avi)

Catalog No. TMPK-00309

Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus. Nectin-4 Protein IgV Domain, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 15.8 kDa and the accession number is Q96NY8-1.

Nectin-4 Protein IgV Domain, Human, Recombinant (His & Avi)

Catalog No. TMPK-00309

Pack Size	Price	Availability
100 μg	297 €	7-10 days
500 μg	1.197 €	7-10 days
1 mg	1.985 €	7-10 days

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Product Information

Biological Activity	Immobilized Human Nectin-4 IgV Domain, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA.
Description	Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus. Nectin-4 Protein IgV Domain, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 15.8 kDa and the accession number is Q96NY8-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	Q96NY8-1
Synonyms	PVRL4,PRR4,NECTIN4,LNIR,EDSS1
Construction	Gly32-Leu146
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	15.8 kDa (predicted). Due to glycosylation, the protein migrates to 16-20 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.