1. Loaded Human Nectin-4-mFc on AMC Biosensor, can bind Human Nectin-1-His with an affinity constant of 27.7 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human Nectin-1-His at 5μg/ml (100 μl/well) can bind Human Nectin-4-mFc . The ED50 of Human Nectin-4-mFc is 1.7 ug/ml. (Regularly tested)

Nectin-4 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with C-mFc tag. The predicted molecular weight is 80-90 KDa and the accession number is Q96NY8.

Human

HEK293 Cells

PVRL4,PRR4,Poliovirus receptor-related protein 4,Nectin-4,LNIR,Ig superfamily receptor LNIR

Gly32-Val351

Gly32-Val351

Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Nectin-4 (PVRL4) is a type I transmembrane glycoprotein which belongs to the nectin family of Ig superfamily proteins. It contains two Ig-like C2-type domains and one Ig-like V-type domain. PVRL4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with nectin-1. It does not act as receptor for alpha-herpesvirus entry into cells. It is predominantly expressed in placenta, the embryo and breast carcinoma. But it is not detected in normal breast epithelium. The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder.