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NKG2, also known as NKG2A(CD159A), is a member of the killer cell lectin-like receptor family. NKG2A marks a unique immune effector subset preferentially co-expressing the tissue-resident CD103 molecule, but not immune checkpoint inhibitors.It is expressed only in NK-cells, but not in T-cells or B-cells. NKG2A/CD159a Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 18.3 kDa and the accession number is P26715-1.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $487 | 7-10 days | |
500 μg | $1,950 | 7-10 days | |
1 mg | $3,250 | 7-10 days |
Biological Activity | 1. Immobilized Human NKG2A, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 0.51μg/ml determined by ELISA (QC Test).
2. Anti-NKG2A antibody captured on Protein A chip, can bind Human NKG2A, His Tag with an affinity constant of 0.23μM as determined in a SPR assay. |
Description | NKG2, also known as NKG2A(CD159A), is a member of the killer cell lectin-like receptor family. NKG2A marks a unique immune effector subset preferentially co-expressing the tissue-resident CD103 molecule, but not immune checkpoint inhibitors.It is expressed only in NK-cells, but not in T-cells or B-cells. NKG2A/CD159a Protein, Human, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with N-His-Avi tag. The predicted molecular weight is 18.3 kDa and the accession number is P26715-1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | N-His-Avi |
Accession Number | P26715-1 |
Synonyms | NKG2-A,NKG2A,KP43,KLRD1,KLRC1,CD94,CD159A |
Construction | Arg100-Leu233 |
Protein Purity | > 90% as determined by Tris-Bis PAGE |
Molecular Weight | 18.3 kDa (predicted). Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | NKG2, also known as NKG2A(CD159A), is a member of the killer cell lectin-like receptor family. NKG2A marks a unique immune effector subset preferentially co-expressing the tissue-resident CD103 molecule, but not immune checkpoint inhibitors.It is expressed only in NK-cells, but not in T-cells or B-cells. |
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