PD-1 Protein (Primary Amine Labeling), Mouse, Recombinant (hFc), Biotinylated

Catalog No. TMPK-00181

Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity. PD-1 Protein (Primary Amine Labeling), Mouse, Recombinant (hFc), Biotinylated is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 42.9 kDa and the accession number is Q02242.

PD-1 Protein (Primary Amine Labeling), Mouse, Recombinant (hFc), Biotinylated

Catalog No. TMPK-00181

Pack Size	Price	Availability
100 μg	$814	7-10 days
500 μg	$3,250	7-10 days
1 mg	$5,440	7-10 days

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Product Information

Biological Activity	Mouse PD-L1, His Tag captured on CM5 Chip via anti-his antibody can bind Biotinylated Mouse PD-1, hFc Tag with an affinity constant of 3.35 μM as determined in SPR assay.
Description	Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity. PD-1 Protein (Primary Amine Labeling), Mouse, Recombinant (hFc), Biotinylated is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 42.9 kDa and the accession number is Q02242.
Species	Mouse
Expression System	HEK293 Cells
Tag	C-hFc
Accession Number	Q02242
Synonyms	SLEB2,PDCD1,PD-1,PD1,CD279
Construction	Leu25-Gln167
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	42.9 kDa (predicted). Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.