PD-L1 Protein, Human, Recombinant (aa 19-238, His & Avi), Biotinylated

Catalog No. TMPK-00182

B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1 Protein, Human, Recombinant (aa 19-238, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.1 kDa and the accession number is Q9NZQ7-1.

PD-L1 Protein, Human, Recombinant (aa 19-238, His & Avi), Biotinylated

Catalog No. TMPK-00182

Pack Size	Price	Availability
100 μg	$814	7-10 days
500 μg	$3,250	7-10 days
1 mg	$5,440	7-10 days

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Product Information

Biological Activity	Immobilized Anti-PD-L1 Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human PD-L1, His Tag with the EC50 of 9.4ng/ml determined by ELISA.
Description	B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1 Protein, Human, Recombinant (aa 19-238, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.1 kDa and the accession number is Q9NZQ7-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	Q9NZQ7-1
Synonyms	PD-L1B7 homolog 1,PD-L1,PDL1,PDCD1LG1,PDCD1L1,CD274,B7-H1,B7H1,B7-H
Construction	Phe19-Arg238
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	28.1 kDa (predicted). Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.