RANKL/TNFSF11/CD254 Protein, Human, Recombinant (aa 63-244)

Catalog No. TMPK-00716

Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone. RANKL/TNFSF11/CD254 Protein, Human, Recombinant (aa 63-244) is expressed in HEK293 mammalian cells. The predicted molecular weight is 20.5 kDa and the accession number is O14788-2.

RANKL/TNFSF11/CD254 Protein, Human, Recombinant (aa 63-244)

Catalog No. TMPK-00716

Pack Size	Price	Availability
100 μg	$581	7-10 days
500 μg	$2,330	7-10 days
1 mg	$3,880	7-10 days

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Product Information

Biological Activity	1. Immobilized Human RANKL, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 13.6ng/ml determined by ELISA (QC Test). 2. Human TNFRSF11B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human RANKL, No Tag with an affinity constant of 0.030 nM as determined in SPR assay.
Description	Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone. RANKL/TNFSF11/CD254 Protein, Human, Recombinant (aa 63-244) is expressed in HEK293 mammalian cells. The predicted molecular weight is 20.5 kDa and the accession number is O14788-2.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	O14788-2
Synonyms	TRANCE,TNFSF11,sOdf,RANKL,OPTB2,OPGL,ODF,CD254
Construction	Gly63-Asp244
Protein Purity	> 90% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
Molecular Weight	20.5 kDa (predicted). Due to glycosylation, the protein migrates to 30-60 kDa based on Tris-Bis PAGE resu
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Receptor activator of nuclear factor x03BA;B (RANK) and its ligand (RANKL) have originally been described for their key roles in bone metabolism and the immune system. Subsequently, it has been shown that the RANKL-RANK system is critical in the formation of mammary epithelia in lactating females and the thermoregulation of the central nervous system. RANKL and RANK are under the tight control of the female sex hormones estradiol and progesterone.