SIRP alpha V6 Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPK-01364

Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction. SIRP alpha V6 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 39.9 kDa and the accession number is P78324 variant 6.

SIRP alpha V6 Protein, Human, Recombinant (His & Avi), Biotinylated

Catalog No. TMPK-01364

Pack Size	Price	Availability
100 μg	885 €	7-10 days
500 μg	3.534 €	7-10 days
1 mg	5.899 €	7-10 days

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Product Information

Biological Activity	Immobilized Biotinylated Human SIRP alpha V6, His Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 0.21/μg/ml determined by ELISA.
Description	Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction. SIRP alpha V6 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 39.9 kDa and the accession number is P78324 variant 6.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	P78324 variant 6
Synonyms	SIRPA,SIRP α V6,SIRP α,SIRP alpha,SHPS-1,SHPS1,PTPNS1,P84,MYD-1,MYD1,MFR,CD172a,BIT
Construction	Glu31-Arg370(S105P)
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	39.9 kDa (predicted). Due to glycosylation, the protein migrates to 60-65 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.