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Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $814 | 7-10 days | |
500 μg | $3,250 | 7-10 days | |
1 mg | $5,440 | 7-10 days |
Biological Information | Immobilized Human CD47, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human SIRP alpha V8, His Tag with the EC50 of 0.53ug/ml determined by ELISA. |
Description | Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction. SIRP alpha V8 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 40.1 kDa and the accession number is ATD50864.1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-His-Avi |
Accession Number | ATD50864.1 |
Synonyms | P84,MFR,MYD1,SHPS-1,SIRP alpha,SIRP α,SIRPα,MYD-1,PTPNS1,SIRP α V8,SHPS1,CD172a,BIT,SIRPA |
Construction | Glu31-Arg369(S44L, S50T, I52T, H54R, V57A) |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC |
Molecular Weight | 40.1 kDa (predicted). Due to glycosylation, the protein migrates to 52-68 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction. |
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