2,4,6-Trimethylphenol (Mesitol), primarily reacting with organic matter (3 DOM*), is a probe compound that undergoes rapid oxidation by singlet oxygen in aqueous solutions.

Peroxidatic substrates, namely catechol (CAT) and 2,4,6-trimethylphenol (TMP) were employed to investigate the chloride-responsive reactions facilitated by chloroperoxidase (CPO). TMP demonstrates consumption exclusively in the presence of chloride ions. While TMP acts as a competitive inhibitor in relation to CAT, the inhibition pattern shifts for CAT in the presence of TMP during chloride-dependent CPO-catalyzed peroxidation reactions. In direct competition studies, the TMP to CAT consumption ratio in the chloride-dependent CPO reaction exhibits an augmentation as the chloride concentration is elevated from 1.0 to 400 mM.[3]