2-Bromohexadecanoic acid (2-BP) is a non-metabolizable palmitate analog, an agonist of PPARδ, which acts as a palmitoylation inhibitor and inhibits DHHC-mediated palmitoylation.

Palmitoylation:

METHODS: HepG2 was treated with PA (200 µM) and 2-Bromohexadecanoic acid (25-150 µM) to examine CSC sphere morphology and number.
RESULTS: 2-Bromohexadecanoic acid (25, 50 and 150 µM) significantly reduced PA-induced CSC sphere formation in HepG2 (75-150 µM and >150 µM.[1])
METHODS: ERα or ERα-Cys447Ala-transfected HeLa and HepG2 cells were pretreated with 2-Bromohexadecanoic acid (10 µM) for 30 min, then stimulated with E2 (10 nM) for 10 min, and the target protein expression levels were detected by Western Blot.
RESULTS: E2 induced ERK and AKT phosphorylation in ERα-transfected HeLa and HepG2 cells. E2-induced signaling kinase activation was completely blocked without affecting the basal level of phosphorylation if the cells were pretreated with the PAT inhibitor 2-Bromohexadecanoic acid.E2 was ineffective in inducing phosphorylation of ERK and AKT in ERα-Cys447Ala mutant-transfected HeLa cells. [2]