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ADU-S100 disodium salt

Catalog No. T10252 CAS 1638750-95-4

Synonyms: ML RR-S2 CDA disodium salt, MIW815 disodium salt

ADU-S100 (MIW815) disodium salt is an activator of stimulator of interferon genes (STING).

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ADU-S100 disodium salt Chemical Structure

ADU-S100 disodium salt, CAS 1638750-95-4

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Description	ADU-S100 (MIW815) disodium salt is an activator of stimulator of interferon genes (STING).
In vitro	ADU-S100 shows enhanced type I IFN production over CDA in THP-1 human monocytes. ADU-S100 induces the highest expression of IFN-β and the pro-inflammatory cytokines TNF-α, IL-6, and MCP-1 on a molar equivalent basis, as compared to endogenous ML cGAMP and the TLR3 agonist poly I:C. ADU-S100 is also found to induce aggregation of STING and induce phosphorylation of TBK1 and IRF3 in mouse bone marrow macrophage (BMM). ADU-S100 induces significantly higher levels of IFN-α when compared to ML cGAMP.
In vivo	ADU-S100 demonstrates superior anti-tumor efficacy compared to the endogenous molecule ML cGAMP. Upon conducting a dose-response analysis in B16 tumor-bearing mice with the ADU-S100 compound, an optimal dose for maximal antitumor activity was identified. This optimal dose not only elicits heightened tumor antigen-specific CD8+ T cell responses but also significantly enhances long-term survival rates to 50%.
Cell Research	Cryopreserved hPBMCs are thawed and 1×10^6 cells per well are plated in a 96 well plate in RPMI media supplemented with 10% FBS, 1% non-essential amino acids, 1% penicillin/streptomycin, L-glutamine, 10 mM HEPES buffer, 1 mM Sodium Pyruvate, 0.055 mM β-ME at 37°C with 5% CO2. Cells are stimulated with 10 μM ADU-S100 or ML cGAMP for 6 hours and supernatants are harvested. Supernatants are diluted 1:2 and assayed for IFN-α protein using Cytometric Bead Array (CBA) Human Flex Set. Data is collected using a FACSVerse cytometer.
Animal Research	WT C57BL/6 mice are inoculated with 5×10^4 B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm3 mice receive three IT doses of either ML RR-S2 CDG (25 μg), ADU-S100 (50 μg), or HBSS as control. WT C57BL/6 mice are inoculated with 5×10^4 B16.F10 cells in the left flank (n=5). When tumor volumes are 100 mm3 they received three IT doses of ADU-S100 at 5, 25, 50, or 100 μg or HBSS as control. WT C57BL/6 mice are inoculated with 5×104 B16.F10 cells in the left flank (n=8). When tumor volumes are 100 mm3 they receive three IT doses of 100 μg ADU-S100 or HBSS as control. Treatments are administered on days 13, 17, and 20 and tumor measurements are taken twice weekly. Results are shown as percent survival by Log-rank (Mantel-Cox) test (A and C).

Synonyms	ML RR-S2 CDA disodium salt, MIW815 disodium salt
Molecular Weight	736.52
Formula	C20H24N10Na2O10P2S2
CAS No.	1638750-95-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 300 mg/mL (408.44 mM), Sonication is recommended.

References and Literature

1. Corrales L, et al. Direct Activation of STING in the Tumor Microenvironment Leads to Potent and Systemic Tumor Regression and Immunity. Cell Rep. 2015 May 19;11(7):1018-30.

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Keywords

ADU-S100 disodium salt 1638750-95-4 Others ADU-S 100 ML RR-S2 CDA MIW 815 ML RR-S2 CDA disodium salt ADU-S100 ADUS100 disodium salt MIW815 MIW815 disodium ADU S100 disodium salt ADU-S100 disodium ADU-S-100 disodium salt MIW815 disodium salt ML RR-S2 CDA disodium MIW-815 ADU-S-100 inhibitor inhibit

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