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Biotin-VAD-FMK

Biotin-VAD-FMK
Biotin-VAD-FMK is a cell-permeable, irreversible biotin-labeled inhibitor of caspase. It is used to identify active caspases in cell lysates.
Catalog No. T10548Cas No. 1135688-15-1

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Biotin-VAD-FMK

Catalog No. T10548Cas No. 1135688-15-1

Biotin-VAD-FMK is a cell-permeable, irreversible biotin-labeled inhibitor of caspase. It is used to identify active caspases in cell lysates.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
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Product Introduction

Bioactivity
Description
Biotin-VAD-FMK is a cell-permeable, irreversible biotin-labeled inhibitor of caspase. It is used to identify active caspases in cell lysates.
In vitro
Biotin-VAD-FMK can facilitate cell permeability.
Chemical Properties
Molecular Weight672.81
FormulaC30H49FN6O8S
Cas No.1135688-15-1
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 27.3 mg/mL (40.58 mM)
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM1.4863 mL7.4315 mL14.8630 mL74.3152 mL
5 mM0.2973 mL1.4863 mL2.9726 mL14.8630 mL
10 mM0.1486 mL0.7432 mL1.4863 mL7.4315 mL
20 mM0.0743 mL0.3716 mL0.7432 mL3.7158 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
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Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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